Part:BBa_K4800017:Design

MmCAR

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 427
  Illegal PstI site found at 454
  Illegal PstI site found at 1723
  Illegal PstI site found at 1750
  Illegal PstI site found at 2143
  Illegal PstI site found at 2639
  Illegal PstI site found at 3283
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 427
  Illegal PstI site found at 454
  Illegal PstI site found at 1723
  Illegal PstI site found at 1750
  Illegal PstI site found at 2143
  Illegal PstI site found at 2639
  Illegal PstI site found at 3283
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 981
  Illegal BglII site found at 1607
  Illegal BglII site found at 1883
  Illegal BglII site found at 2574
  Illegal XhoI site found at 3523
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 427
  Illegal PstI site found at 454
  Illegal PstI site found at 1723
  Illegal PstI site found at 1750
  Illegal PstI site found at 2143
  Illegal PstI site found at 2639
  Illegal PstI site found at 3283
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 427
  Illegal PstI site found at 454
  Illegal PstI site found at 1723
  Illegal PstI site found at 1750
  Illegal PstI site found at 2143
  Illegal PstI site found at 2639
  Illegal PstI site found at 3283
  Illegal NgoMIV site found at 2251
  Illegal NgoMIV site found at 2313
  Illegal NgoMIV site found at 2530
  Illegal NgoMIV site found at 2977
  Illegal AgeI site found at 157
  Illegal AgeI site found at 1168
  Illegal AgeI site found at 1465
  Illegal AgeI site found at 2350
  Illegal AgeI site found at 2359
  Illegal AgeI site found at 3034
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.

Source

MmCAR is from Mycobacterium marinum.